浜戝崡鏉傝崏绋讳笉鑲插熀鍥犵簿缁嗗畾浣嶏紝鍙戣偛閫斿緞鐨勮〃鍨嬭瀵熷垎鏋愩
鈫 銆愮浉鍏炽戝垬鑰鍏夊凡鍙戠殑PNAS鍚屼釜鍩哄洜锛 
High Temperatures Cause Male Sterility in Rice Plants with Transcriptional Alterations during Pollen Development.聽聽 [涓嬭浇]
楂樻俯浣垮緱閮ㄥ垎鍦ㄨ姳鑽粧姣″眰鐗瑰紓鎬ц〃杈剧殑鍩哄洜琛ㄨ揪姘村钩涓嬭皟锛屼粠鑰岀粧姣″眰姝ｅ父鍔熻兘(鑺辩矇鐨勯粡鍚堝拰鍙戣偛)閬埌鐮村潖锛屾渶缁堝奖鍝嶈偛鎬с
Plant male reproductive development is highly organized and sensitive to various environmental stressors, including high temperature. We have established an experimental procedure to evaluate high temperature injury in japonica rice plants. High temperature treatment (39 degrees C/30 degrees C) starting at the microspore stage repeatedly reduced spikelet fertility in our system. Morphological observations revealed that pollen viability in plants exposed to high temperatures was lower than that in control plants. Most pollen grains in high temperature-treated plants displayed a normal round shape and stained reddish purple with Alexander’s reagent; however, the pollen grains were very poorly attached and displayed limited germination on the stigma. To investigate gene regulatory mechanisms in the anther in high temperature environments, DNA microarray analysis was performed by comparing non-treated samples to samples treated with two to four days of high heat. Genes responsive to high temperatures were identified from clustering of microarray data. Among these, at least thirteen were designated as high temperature -repressed genes in the anther. Expression analyses revealed that these genes were expressed specifically in the immature anther mainly in the tapetum at the microspore stage and down-regulated after one day of high temperature. The expression levels of Osc6, OsRAFTIN and TDR, which are tapetum-specific genes, were unaffected by high temperatures. These results suggest that not all tapetal genes are inhibited by increased heat and the tapetum itself is not degraded in such an environment. However, high temperatures may disrupt some of the tapetum functions required for pollen adhesion and germination on the stigma.
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