鍏充簬鈥�鍩鸿川鍔犲伐鑲介叾鈥�/鑽у厜鏂规硶鏉ヨ瀵熺７鍦ㄦ牴閮ㄧ殑绉诲姩

1. a rice stromal processing peptidase regulates chloroplast and root development

涓�涓��鍩鸿川鍔犲伐鑲介叾鈥濊皟鎺у彾缁夸綋鍜屾牴鐨勫彂鑲层��

2. Dynamics of Periarbuscular Membranes Visualized with a Fluorescent Phosphate Transporter in Arbuscular Mycorrhizal Roots of Rice

妞嶇墿鍜屼笡鏋濊弻鏍瑰叡鐢燂紝妞嶇墿鍏夊悎浣滅敤缁欑湡鑿屼緵搴斿吇鍒嗭紝浣滀负鍥炴姤锛屼粬浠湪鐪熻弻閭ｅ効鍙互鑾峰緱纾烽吀鐩愮瓑鏃犳満鍏诲垎銆傝鏂囩珷鏄敤鑽у厜鏂规硶鏉ヨ瀵熺７鍦ㄦ牴閮ㄧ殑绉诲姩銆備晶閲嶆柟娉曟柟闈€��

锛�1锛�涓庤姳鐩稿叧鐨勫悓婧愬紓鍨嬪熀鍥狅紙2锛�鑼夎帀閰镐俊鍙疯皟鎺у洜瀛怰IM1锛�3锛夎姳绮夌鐢熼暱涓恫娉″垎閫夊彈浣撳拰鍒嗘硨鍨嬭浇浣撹啘铔嬬櫧

1. Functional conservation and diversification of class E floral homeotic genes in rice (Oryza sativa)

涓庤姳鐩稿叧鐨勫悓婧愬紓鍨嬪熀鍥犲姛鑳界殑鐮旂┒銆�

2. The NAC transcription factor RIM1 of rice is a new regulator of jasmonate signaling

鑼夎帀閰镐俊鍙疯皟鎺у洜瀛怰IM1銆�

3. Vacuolar sorting receptors (VSRs) and secretory carrier membrane proteins (SCAMPs) are essential for pollen tube growth

鑺辩矇绠＄敓闀夸腑娑叉场鍒嗛�夊彈浣撳拰鍒嗘硨鍨嬭浇浣撹啘铔嬬櫧鐨勯噸瑕佷綔鐢ㄣ��

Cytochrome P450 Family Member CYP704B2 Catalyzes the {omega}-Hydroxylation of Fatty Acids and Is Required for Anther Cutin Biosynthesis and Pollen Exine Formation in Rice.

缁嗚優鑹茬礌P450瀹舵棌鎴愬憳CYP704B2鍌寲鑴傝偑閰哥殑{omega}-缇熷寲(?)锛屽浜庤姳鑽璐ㄥ舰鎴愬拰鑺辩矇澶栧褰㈡垚鐨勯噸瑕佹�с��

The anther cuticle and microspore exine act as protective barriers for the male gametophyte and pollen grain, but relatively little is known about the mechanisms underlying the biosynthesis of the monomers of which they are composed. We report here the isolation and characterization of a rice (Oryza sativa) male sterile mutant, cyp704B2, which exhibits a swollen sporophytic tapetal layer, aborted pollen grains without detectable exine, and undeveloped anther cuticle. In addition, chemical composition analysis indicated that cutin monomers were hardly detectable in the cyp704B2 anthers. These defects are caused by a mutation in a cytochrome P450 family gene, CYP704B2. The CYP704B2 transcript is specifically detected in the tapetum and the microspore from stage 8 of anther development to stage 10. Heterologous expression of CYP704B2 in yeast demonstrated that CYP704B2 catalyzes the production of -hydroxylated fatty acids with 16 and 18 carbon chains. Our results provide insights into the biosynthesis of the two biopolymers sporopollenin and cutin. Specifically, our study indicates that the -hydroxylation pathway of fatty acids relying on this ancient CYP704B family, conserved from moss to angiosperms, is essential for the formation of both cuticle and exine during plant male reproductive and spore development.

The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice.

PMID: 20039013聽 Theor Appl Genet. 2009 Dec 29.

A single recessive gene controls cadmium translocation in the cadmium hyperaccumulating rice cultivar Cho-Ko-Koku.

PMID: 20039193聽 Plant Mol Biol. 2009 Dec 29.

The bZIP transcription factor OsABF1 is an ABA responsive element binding factor that enhances abiotic stress signaling in rice.

PMID: 20040065聽 Plant Cell Environ. 2009 Dec 23.

ACO1, a gene for aminocyclopropane-1-carboxylate oxidase: effects on internode elongation at the heading stage in rice. 銆愮◢鍊欍��

Advance Access published online on December 29, 2009

Function and Expression Pattern of the {alpha} Subunit of the Heterotrimeric G Protein in Rice <鏈帓鐗�>

姘寸ɑ寮備笁鑱氫綋G铔嬬櫧伪浜氬熀鐨勫姛鑳戒笌琛ㄨ揪銆傝寰楁瘮杈冨ぇ锛屽叾瀹炲氨鏄熀鍥燝鐨勮〃杈俱�丟US涔嬬被鍒嗘瀽锛屾潗鏂欐槸鏌愪釜鐭潌鍦嗙矑鐨勭獊鍙樹綋d1銆�

The d1 mutant which is deficient for the heterotrimeric G protein subunit (G) gene of rice, shows dwarfism and sets small-round seeds. To determine whether dwarfism in d1 is due to a reduction of cell number or to shortened cell length, the cell number of the leaf sheath, the internode, the root and the lemma was compared between Nipponbare, a wild type rice and d1-5, a d1 allele derived from Nipponbare. Our results indicate that the cell number was reduced in all organs analyzed in d1-5. In addition, cell enlargement was found in roots and lemma of d1-5, although the organ length in d1-5 was shorter than that of the wild type rice. These results suggest that rice G participates in cell proliferation in rice. Western blot analyses using antiG antibody and RT-PCR analyses indicate that G is mostly expressed in the developing organs. G promoter activity studies using the GUS reporter gene confirmed that the expression of G was highest in developing organs. We conclude that rice G participates in the regulation of cell number in a developmental stage dependent manner.

Over-expression of miR172 causes loss of spikelet determinacy and floral organ abnormalities in rice (Oryza sativa). <鏈帓鐗�>

灏廟NA鐨勮繃閲忚〃杈撅紝褰卞搷琛ㄥ瀷銆�

J Exp Bot. 2009 Dec 16. [Epub ahead of print]

Overexpression of OsRAN2 in rice and Arabidopsis renders transgenic plants hypersensitive to salinity and osmotic stress.

RAN鐨勮繃閲忚〃杈撅紝褰卞搷鎶楁�с��

1. Mapping and validation of quantitative trait loci for spikelets per panicle and 1,000-grain weight in rice (Oryza sativa L.).聽 [涓嬭浇]

姣忕棰栨暟鍜屽崈绮掗噸鐨凲TL

2. Dissection of a QTL reveals an adaptive, interacting gene complex associated with transgressive variation for flowering time in rice.聽 [涓嬭浇]

QTL鍒嗘瀽鎻ず鎺у埗姘寸ɑ寮�鑺辨椂闂寸殑鍩哄洜澶嶆潅浜掍綔鈥︹��

1. Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed. <鏈帓鐗�>

VPE娑叉场鍔犲伐閰讹紵鍚笂鍘绘尯鐔熸倝鈥︹��

2. Knockdown of OsDCL2 in Rice Negatively Affects Maintenance of the Endogenous dsRNA Virus, Oryza sativa endornavirus. <鏈帓鐗�>

OsDCL2锛屽叧浜庡皬RNA鐨勶紝娌″叴瓒ｇ殑鏃犺鍚с��

3. PANICLE PHYTOMER2 (PAP2), encoding a SEPALLATA subfamily MADS-box protein, positively controls spikelet meristem identity in rice. <鏈帓鐗�>

PAP2锛屾帶鍒剁鍒嗗寲鐨勪竴鍩哄洜锛孧ADS-box铔嬬櫧銆�

ERECT PANICAL2 Encodes a Novel Protein That Regulates Panicle Erectness in Indica Rice. <鏈帓鐗�>

绫肩ɑ涓皟鎺х鐩寸珛鐨勫熀鍥燛P2锛屽熀浜庣鐩寸珛鐨勪袱涓奔绋诲ぉ鐒剁獊鍙樹綋銆傜敓鐞嗗疄楠屻�佹瘮杈冨父瑙勭殑鍩哄洜鍥句綅鍏嬮殕銆�

Rice (Oryza sativa L.) inflorescence (panicle) architecture is an important agronomic trait for rice breeding. A number of high-yielding japonica rice strains, characterized by an erect panicle (EP) of their architecture, have been released as commercial varieties in China. But no EP-type indica varieties are released so far. Here, we identified two allelic erect-panicle mutants in indica rice, erect panicle2-1 (ep2-1) and erect panicle2-2 (ep2-2), exhibiting the characteristic erect panicle phenotype. Both mutants were derived from spontaneous mutation. We cloned the EP2 gene by way of a map-based cloning strategy, and transgenic complementation test rescued the phenotype of the ep2-1. Anatomical investigations revealed that the ep2 mutants have more vascular bundles and thicker stem than that of wild-type plants, explaining the panicle erectness phenotype in ep2 mutants. It was shown that the EP2 was specifically expressed in the vascular bundles of internodes by GUS staining and RT-PCR. The EP2 encodes a novel plant-specific protein, which localizes to the endoplasmic reticulum with unknown biochemical function. In addition, the EP2 also regulates other panicle characteristics, such as panicle length and grain size, but grain number of per panicle has little change, indicating that the mutation of ep2 gene could be applied in the EP-type indica rice breeding.

The expression of Orysa;CycB1;1 is essential for endosperm formation and causes embryo enlargement in rice.聽聽 [涓嬭浇]

缁嗚優鍛ㄦ湡涓庤儦涔冲彂鑲茬殑鍏崇郴锛屾秹鍙婂埌鐨勫熀鍥犳槸CycB1;1銆傜己澶辨甯哥殑璇ュ熀鍥犲鑷磋儦寮傚父澧炲ぇ锛屽師鍥犳槸缁嗚優鍛ㄦ湡绱婁贡銆佸鑷翠笉姝ｅ父鏈変笣鍒嗚銆佸鑷寸粏鑳炲澶�(鑰屼笉鏄澶�)銆傚仛浜嗚〃杈炬ā寮� & Genome-wide transcriptional profiling銆�

The cell cycle is an important process during seed development in plants and its progression is driven by a number of core regulators such as the cyclins. Currently, however, little is known regarding the role of the cyclins in embryo and endosperm development in cereals. In our current study, we show that the knockdown of Orysa;CycB1;1 in rice results in the production of abnormal seeds, which at maturity contain only an enlarged embryo. It was further found that a delayed and abnormal cellularization occurred in the endosperm in these knockdown seeds which eventually became abortive. Moreover, the observed development of the enlarged embryo was also morphologically abnormal and found to be caused by an enlarged cell size rather than an increased cell number. Expression analysis showed that Orysa;CycB1;1 transcripts were localized in the endosperm and embryo. Genome-wide transcriptional profiling further indicated that a large number of genes are responsible for the phenotype of the enlarged embryo. The results of the knockdown of Orysa;CycB1;1 via an endosperm or an embryo-specific promoter also suggest that the enlarged embryo may be correlated to the abortive endosperm. Our results suggest that Orysa;CycB1;1 expression is critical for endosperm formation via the regulation of mitotic division, and that the endosperm plays an important role in maintenance of embryo development in rice.