鍏充簬鈥�鍩鸿川鍔犲伐鑲介叾鈥�/鑽у厜鏂规硶鏉ヨ瀵熺７鍦ㄦ牴閮ㄧ殑绉诲姩

1. a rice stromal processing peptidase regulates chloroplast and root development

涓�涓��鍩鸿川鍔犲伐鑲介叾鈥濊皟鎺у彾缁夸綋鍜屾牴鐨勫彂鑲层��

2. Dynamics of Periarbuscular Membranes Visualized with a Fluorescent Phosphate Transporter in Arbuscular Mycorrhizal Roots of Rice

妞嶇墿鍜屼笡鏋濊弻鏍瑰叡鐢燂紝妞嶇墿鍏夊悎浣滅敤缁欑湡鑿屼緵搴斿吇鍒嗭紝浣滀负鍥炴姤锛屼粬浠湪鐪熻弻閭ｅ効鍙互鑾峰緱纾烽吀鐩愮瓑鏃犳満鍏诲垎銆傝鏂囩珷鏄敤鑽у厜鏂规硶鏉ヨ瀵熺７鍦ㄦ牴閮ㄧ殑绉诲姩銆備晶閲嶆柟娉曟柟闈€��

锛�1锛�涓庤姳鐩稿叧鐨勫悓婧愬紓鍨嬪熀鍥狅紙2锛�鑼夎帀閰镐俊鍙疯皟鎺у洜瀛怰IM1锛�3锛夎姳绮夌鐢熼暱涓恫娉″垎閫夊彈浣撳拰鍒嗘硨鍨嬭浇浣撹啘铔嬬櫧

1. Functional conservation and diversification of class E floral homeotic genes in rice (Oryza sativa)

涓庤姳鐩稿叧鐨勫悓婧愬紓鍨嬪熀鍥犲姛鑳界殑鐮旂┒銆�

2. The NAC transcription factor RIM1 of rice is a new regulator of jasmonate signaling

鑼夎帀閰镐俊鍙疯皟鎺у洜瀛怰IM1銆�

3. Vacuolar sorting receptors (VSRs) and secretory carrier membrane proteins (SCAMPs) are essential for pollen tube growth

鑺辩矇绠＄敓闀夸腑娑叉场鍒嗛�夊彈浣撳拰鍒嗘硨鍨嬭浇浣撹啘铔嬬櫧鐨勯噸瑕佷綔鐢ㄣ��

鈥極mics鈥� analyses of regulatory networks in plant abiotic stress responses

RT锛岀敤缁勫鏂规硶鐮旂┒闈炵敓鐗╄儊杩殑缁艰堪

鍙︼細Plant Physiology Preview
Published on January 15, 2010; 10.1104/pp.109.149872

Characterization of the rice PHO1 gene family reveals a key role forOsPHO1;2 in phosphate homeostasis and the evolution of a distinct clade indicotyledons (鏈帓鐗�)

姘寸ɑPHO1鍩哄洜鍦ㄧ７閰哥洂鍔ㄦ�佸钩琛′腑鐨勪綔鐢紝浠ュ強鍩哄洜杩涘寲鏂归潰鐨勭爺绌躲��

鈭毬� 鍐嶉檮涓�绡囦笉鐩稿叧鐨勬枃锛�A Comparison of the Effects of Three GM Corn Varieties on Mammalian Health(html鍏ㄦ枃)

鏍规嵁鍙戣〃鍦�2009骞�12鏈堜竴鏈熴��鐢熺墿绉戝鍥介檯鏈熷垔銆嬩笂鐨勭爺绌讹紝涓夌瀛熷北閮藉叕鍙哥殑杞熀鍥犵帀绫宠兘璁╄�侀紶鐨勮倽鑴忋�佽偩鑴忓拰鍏跺畠鍣ㄥ畼鍙楁崯銆備笁绉嶈浆鍩哄洜鐜夌背鍝佺锛屼竴绉嶈璁¤兘鎶楀箍璋遍櫎鑽夊墏锛堝嵆鎵�璋撶殑Roundup-ready锛夛紝鍙﹀涓ょ鍚湁缁嗚弻琛嶇敓铔嬬櫧璐紝鍏锋湁鏉�铏墏鐗规�с�傝繖椤圭爺绌跺埄鐢ㄤ簡瀛熷北閮借嚜宸辩殑鍘熷鏁版嵁銆�

The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice.

PMID: 20039013聽 Theor Appl Genet. 2009 Dec 29.

A single recessive gene controls cadmium translocation in the cadmium hyperaccumulating rice cultivar Cho-Ko-Koku.

PMID: 20039193聽 Plant Mol Biol. 2009 Dec 29.

The bZIP transcription factor OsABF1 is an ABA responsive element binding factor that enhances abiotic stress signaling in rice.

PMID: 20040065聽 Plant Cell Environ. 2009 Dec 23.

ACO1, a gene for aminocyclopropane-1-carboxylate oxidase: effects on internode elongation at the heading stage in rice. 銆愮◢鍊欍��

Theor Appl Genet. 2009 Dec 25. [Epub ahead of print]

Development and application of gene-based markers for the major rice QTL Phosphorus uptake 1.

纾峰惛鏀禥TL鐨勬娴嬪拰搴旂敤銆�

Marker-assisted breeding is a very useful tool for breeders but still lags behind its potential because information on the effect of quantitative trait loci (QTLs) in different genetic backgrounds and ideal molecular markers are unavailable. Here, we report on some first steps toward the validation and application of the major rice QTL Phosphate uptake 1 (Pup1) that confers tolerance of phosphorus (P) deficiency in rice (Oryza sativa L.). Based on the Pup1 genomic sequence of the tolerant donor variety Kasalath that recently became available, markers were designed that target (1) putative genes that are partially conserved in the Nipponbare reference genome and (2) Kasalath-specific genes that are located in a large insertion-deletion (INDEL) region that is absent in Nipponbare. Testing these markers in 159 diverse rice accessions confirmed their diagnostic value across genotypes and showed that Pup1 is present in more than 50% of rice accessions adapted to stress-prone environments, whereas it was detected in only about 10% of the analyzed irrigated/lowland varieties. Furthermore, the Pup1 locus was detected in more than 80% of the analyzed drought-tolerant rice breeding lines, suggesting that breeders are unknowingly selecting for Pup1. A hydroponics experiment revealed genotypic differences in the response to P deficiency between upland and irrigated varieties but confirmed that root elongation is independent of Pup1. Contrasting Pup1 near-isogenic lines (NILs) were subsequently grown in two different P-deficient soils and environments. Under the applied aerobic growth conditions, NILs with the Pup1 locus maintained significantly higher grain weight plant(-1) under P deprivation in comparison with intolerant sister lines without Pup1. Overall, the data provide evidence that Pup1 has the potential to improve yield in P-deficient and/or drought-prone environments and in diverse genetic backgrounds.

Published on December 22, 2009; 10.1105/tpc.109.066902

Orthologs of the Class A4 Heat Shock Transcription Factor HsfA4a Confer Cadmium Tolerance in Wheat and Rice

鐑嚮杞綍鍥犲瓙HsfA4a鍙互鎻愰珮灏忛害鍜屾按绋荤殑鑰愰晧銆�

Cadmium (Cd) is a widespread soil pollutant; thus, the underlying molecular controls of plant Cd tolerance are of substantial interest. A screen for wheat (Triticum aestivum) genes that confer Cd tolerance to a Cd hypersensitive yeast strain identified Heat shock transcription factor A4a (HsfA4a). Ta HsfA4a is most similar to the class A4 Hsfs from monocots. The most closely related rice (Oryza sativa) homolog, Os HsfA4a, conferred Cd tolerance in yeast, as did Ta HsfA4a, but the second most closely related rice homolog, Os HsfA4d, did not. Cd tolerance was enhanced in rice plants expressing Ta HsfA4a and decreased in rice plants with knocked-down expression of Os HsfA4a. An analysis of the functional domain using chimeric proteins constructed from Ta HsfA4a and Os HsfA4d revealed that the DNA binding domain (DBD) of HsfA4a is critical for Cd tolerance, and within the DBD, Ala-31 and Leu-42 are important for Cd tolerance. Moreover, Ta HsfA4a鈥搈ediated Cd resistance in yeast requires metallothionein (MT). In the roots of wheat and rice, Cd stress caused increases in HsfA4a expression, together the MT genes. Our findings thus suggest that HsfA4a of wheat and rice confers Cd tolerance by upregulating MT gene expression in planta.

A major quantitative trait locus for increasing cadmium-specific concentration in rice grain is located on the short arm of chromosome 7.

Yano鐨勪竴绡囧皬鏂囩珷锛屽叧浜庢按绋荤瀛愰晧绉疮鐨凲TL鈥︹��

Large phenotypic variations in the cadmium (Cd) concentration of rice grains and shoots have been observed. However, the genetic control of Cd accumulation remains poorly understood. Quantitative trait loci (QTLs) determining the grain Cd concentration of rice grown in a Cd-polluted paddy field were identified. Using a mapping population consisting of 85 backcross inbred lines derived from a cross between the low-Cd-accumulating cultivar Sasanishiki (japonica) and high-Cd-accumulating cultivar Habataki (indica), two QTLs for increasing grain Cd concentration were found on chromosomes 2 and 7. A major-effect QTL, qGCd7 (QTL for grain Cd on chromosome 7), was detected on the short arm of chromosome 7. It accounted for 35.5% of all phenotypic variance in backcross inbred lines. qGCd7 was not genetically related to any QTLs for concentrations of essential trace metals (Cu, Fe, Mn, and Zn) or those for agronomic traits such as heading date, suggesting that this QTL is specific to Cd. Furthermore, the existence of qGCd7 was confirmed using chromosome segment substitution lines (CSSLs) and an F(2) population from a cross between the target CSSL and Sasanishiki grown in a Cd-polluted paddy soil. To our knowledge, qGCd7 is a novel QTL with major effects for increasing grain Cd concentrations.

Published on December 15, 2009; 10.1105/tpc.109.070441

Antagonistic HLH/bHLH Transcription Factors Mediate Brassinosteroid Regulation of Cell Elongation and Plant Development in Rice and Arabidopsis

锛堝摜娣讳釜鍥剧墖鏄笉鏄瘮杈冨舰璞★紵锛変竴鍙剁墖鍊捐鐨勭獊鍙樹綋ili1-D锛孖LI1鍩哄洜缂栫爜bHLH杞綍鍥犲瓙锛屼笌鎷熷崡鑺RE1鍚屾簮銆傚熀鍥犲彈BR璋冩帶琛ㄨ揪锛屽彟澶栫爺绌跺叾涓�IBH1銆�BZR1涔嬮棿鐨勭浉浜掑叧绯汇��

鐢熺墿璋风殑璇︾粏浠嬬粛

In rice (Oryza sativa), brassinosteroids (BRs) induce cell elongation at the adaxial side of the lamina joint to promote leaf bending. We identified a rice mutant (ili1-D) showing an increased lamina inclination phenotype similar to that caused by BR treatment. The ili1-D mutant overexpresses an HLH protein homologous to Arabidopsis thaliana Paclobutrazol Resistance1 (PRE1) and the human Inhibitor of DNA binding proteins. Overexpression and RNA interference suppression of ILI1 increase and reduce, respectively, rice laminar inclination, confirming a positive role of ILI1 in leaf bending. ILI1 and PRE1 interact with basic helix-loop-helix (bHLH) protein IBH1 (ILI1 binding bHLH), whose overexpression causes erect leaf in rice and dwarfism in Arabidopsis. Overexpression of ILI1 or PRE1 increases cell elongation and suppresses dwarf phenotypes caused by overexpression of IBH1 in Arabidopsis. Thus, ILI1 and PRE1 may inactivate inhibitory bHLH transcription factors through heterodimerization. BR increases the RNA levels of ILI1 and PRE1 but represses IBH1 through the transcription factor BZR1. The spatial and temporal expression patterns support roles of ILI1 in laminar joint bending and PRE1/At IBH1 in the transition from growth of young organs to growth arrest. These results demonstrate a conserved mechanism of BR regulation of plant development through a pair of antagonizing HLH/bHLH transcription factors that act downstream of BZR1 in Arabidopsis and rice.

A Raf-like MAPKKK gene DSM1 mediates drought resistance through ROS scavenging in rice <鏈帓鐗�>

MAPK(涓濊鍘熸椿鍖栬泲鐧芥縺閰�)绫诲熀鍥燚SM1鍙備笌姘寸ɑ涓椿鎬ф哀鐨勬竻闄わ紝鍦ㄦ姉鏃变腑璧蜂竴瀹氫綔鐢ㄣ�傚埄鐢ㄧ獊鍙樹綋鍋氱殑锛岃澶氱敓鐞嗘寚鏍囩殑娴嬪畾锛屽共鎵般�佽繃琛ㄨ揪銆佽〃杈惧垎鏋愮瓑绛夈��

Mitogen-activated protein kinase (MAPK) cascades have been identified in various signaling pathways involved in plant development and stress responses. We identified a drought hypersensitive mutant (dsm1) of a putative MAPK kinase kinase (MAPKKK) gene in rice. Two allelic dsm1 mutants were more sensitive than wild type plants to drought stress at both seedling and panicle development stages. The dsm1 mutants lost water more rapidly than wild-type plants under drought stress, which was in agreement with the increased drought sensitivity phenotype of the mutant plants. DSM1-RNA interference lines were also hypersensitive to drought stress. The predicted DSM1 protein belongs to a B3 subgroup of plant Raf-like MAPKKKs and was localized in the nucleus. By real-time PCR analysis, the DSM1 gene was induced by salt, drought, and abscisic acid, but not by cold. Microarray analysis revealed that two peroxidase (POX) genes, POX22.3 and POX8.1, were sharply down-regulated compared to wild type, suggesting that DSM1 may be involved in reactive oxygen species (ROS) signaling. Peroxidase activity, electrolyte leakage, chlorophyll content, and 3,3′-diaminobenzidine staining revealed that the dsm1 mutant was more sensitive to oxidative stress due to an increase in ROS damage caused by the reduced POX activity. Overexpression of DSM1 in rice increased the tolerance to dehydration stress at the seedling stage. Together, these results suggest that DSM1 might be a novel MAPKKK functioning as an early signaling component in regulating responses to drought stress by regulating scavenging of ROS in rice.

Proteomics shed light on the brassinosteroid signaling mechanisms

缁嗚優淇″彿鐨勯噸瑕侀�斿緞锛�娌硅彍绱犲唴閰疊R鐨勭患杩般�傜患杩板叾閫斿緞(complete pathway from an RLK to transcription factors in plants)銆佽川璋变箣绫诲垎鏋愮殑纾烽吀鍖栬皟鎺у厓浠躲�佸悇绉嶈泲鐧界粍瀛︾殑鐮旂┒绛夌瓑銆�

Large numbers of receptor-like kinases (RLKs) play key roles in plant development and defense by perceiving extracellular signals. The mechanisms of ligand-induced kinase activation and downstream signal transduction have been studied for only a few RLK pathways, among which the brassinosteroid (BR) pathway is the best characterized. Recently, proteomics studies identified new components that bridge the last gap in the genetically defined BR-signaling pathway, establishing the first complete pathway from an RLK to transcription factors in plants. Furthermore, analyses of phosphorylation events, mostly by mass spectrometry, provided insights into the mechanistic details of receptor kinase activation and regulation of downstream components by phosphorylation. This review focuses on recent progress in understanding BR signal transduction made by proteomics studies.