Enhancement of fruit shelf life by suppressing N-glycan processing enzymes 
浣滆呭彂鐜帮紝鐣寗閲岄潰鐨勪袱涓熀鍥犖憋紞鐢橀湶绯栬嫹閰讹紙伪锛峂an锛夊拰尾锛嶆皑鍩哄繁绯栬嫹閰讹紙尾锛岺ex锛変績杩涚暘鑼勬灉瀹炵殑鎴愮啛杞寲锛岃繖涓や釜鍩哄洜鐨凴NAi妞嶇墿鏄庢樉瑕佹瘮閲庣敓鍨嬭愪繚瀛1涓湀涔嬩箙锛岃岃秴琛ㄨ揪鐨勬鏍垯鍔犲揩浜嗘垚鐔熻蒋鍖栵紝涓旇繖涓や釜鍩哄洜鍙楁鐗╂垚鐔熸墍闇婵绱犱箼鐑殑璇卞锛岃浆褰曡氨姣旇緝鍙戠幇鍦≧NAi鐨勬鏍噷锛屼竴浜涚粏鑳炲闄嶈В鍜屾灉瀹炴垚鐔熺浉鍏崇殑鍩哄洜琛ㄨ揪涓嬭皟銆
In a globalized economy, the control of fruit ripening is of strategic importance because excessive softening limits shelf life. Efforts have been made to reduce fruit softening in transgenic tomato through the suppression of genes encoding cell wall鈥揹egrading proteins. However, these have met with very limited success. N-glycans are reported to play an important role during fruit ripening, although the role of any particular enzyme is yet unknown. We have identified and targeted two ripening-specific N-glycoprotein modifying enzymes, 伪-mannosidase (伪-Man) and 尾-D-N-acetylhexosaminidase (尾-Hex). We show that their suppression enhances fruit shelf life, owing to the reduced rate of softening. Analysis of transgenic tomatoes revealed 鈮2.5- and 鈮2-fold firmer fruits in the 伪-Man and 尾-Hex RNAi lines, respectively, and 鈮30 days of enhanced shelf life. Overexpression of 伪-Man or 尾-Hex resulted in excessive fruit softening. Expression of 伪-Man and 尾-Hex is induced by the ripening hormone ethylene and is modulated by a regulator of ripening, rin (ripening inhibitor). Furthermore, transcriptomic comparative studies demonstrate the down-regulation of cell wall degradation- and ripening-related genes in RNAi fruits. It is evident from these results that N-glycan processing is involved in ripening-associated fruit softening. Genetic manipulation of N-glycan processing can be of strategic importance to enhance fruit shelf life, without any negative effect on phenotype, including yield.
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1.
Cell 136, 136鈥148, January 9, 2009
Two Novel GPCR-Type G Proteins Are Abscisic Acid Receptors in Arabidopsis聽聽 
鎷熷崡鑺ヤ袱涓狝BA鍙椾綋GTG1銆丟TG2
2.
Annu. Rev. Plant Biol. 2008. 59:387鈥415
Molecular Aspects of Seed Dormancy聽聽
鍏充簬绉嶅瓙浼戠湢鐨勭患杩
]]>Receptor endocytosis and signaling in plants聽聽 [涓嬭浇]
缁嗚優鍐呭悶浣滅敤涓庝俊鍙蜂紶瀵肩殑缁艰堪銆傛彁鍒版鐗╂湁寰堝缁嗚優琛ㄩ潰鍙椾綋婵閰(RLKs)锛岀敱姝ゅ舰鎴愮殑缁嗚優浼犲淇″彿鐨勫鏉傞斿緞锛屽崗璋冪敓鐗╀綋鐨勫垎鍖栫敓闀夸互鍙婃洿濂藉湴閫傚簲鐜銆
The emerging complexity of plant endocytic systems puts it on a par with their animal counterparts, reflecting an essential role in signal transduction. The endocytic machinery regulates the space and the time of signal transduction and processing in the cell. Plants possess numerous cell surface receptor-like kinases (RLKs) (more than 600 members in Arabidopsis thaliana and 1100 in rice), a trend attributed to their indeterminate mode of growth, the absence of cell migration, and the need for adaptation towards the environment. Thus, plants would require a robust and highly plastic endocytic system in order to integrate multiple signaling cues from neighboring cells as well as the environment. Although a comprehensive understanding of how plant endocytosis impacts signaling pathways is still lacking, experimental evidence suggests that both plant and animal endosomal systems extensively control signaling.
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