鈥極mics鈥 analyses of regulatory networks in plant abiotic stress responses 
RT锛岀敤缁勫鏂规硶鐮旂┒闈炵敓鐗╄儊杩殑缁艰堪
鍙︼細Plant Physiology Preview
Published on January 15, 2010; 10.1104/pp.109.149872
姘寸ɑPHO1鍩哄洜鍦ㄧ７閰哥洂鍔ㄦ佸钩琛′腑鐨勪綔鐢紝浠ュ強鍩哄洜杩涘寲鏂归潰鐨勭爺绌躲
鈭毬 鍐嶉檮涓绡囦笉鐩稿叧鐨勬枃锛A Comparison of the Effects of Three GM Corn Varieties on Mammalian Health(html鍏ㄦ枃)
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]]>姝ｅ湪鐪嬶紝鍒嗕韩缁欏ぇ瀹躲傝鍙ラ澶栬瘽锛孏oogleBook纭疄鏄釜濂戒笢瑗匡紝铏界劧瀹冮伃鍙楃潃姝ｄ箟浜哄＋鎵璋撶増鏉冪殑鎺ц瘔銆
A Simple Cipher Governs DNA Recognition by TAL Effectors
Breaking the Code of DNA-Binding Specificity of TAL-Type III Effectors
鎴戜滑鐭ラ亾锛岀梾鍘熻弻浼氶氳繃涓夊瀷鍒嗘硨绯荤粺灏唀ffector铔嬬櫧娉ㄥ叆妞嶇墿缁嗚優鍐呰揪鍒拌嚧鐥呯洰鐨勶紝Xanthomonas锛堥粍鍗曡優鏉嗚弻锛夋湁涓绉峞ffector铔嬬櫧鏄疶AL (transcription activator-like) effectors锛屼粬鍙互閫氳繃缁撳悎骞舵縺娲绘鐗╀綋鍐呯殑鍩哄洜杈惧埌鑷寸梾鐩殑銆傝繖绉嶈泲鐧芥湁34涓皑鍩洪吀搴忓垪姣旇緝閲嶈锛屽叾涓12浣嶅拰13浣嶇殑娈嬪熀鏈夌潃澶氭佹э紝鎴戜滑鎶婅繖绉嶅鎬佹хО浣渞epeat variable-diresidue (RVD)锛岄氳繃鐮旂┒鍙戠幇RVD鍐冲畾浜嗗畠鎵缁撳悎鐨凞NA搴忓垪锛屼竴绉峈VD瀵瑰簲涓绉嶆牳鑻烽吀搴忓垪锛屽苟涓斿叿鏈夊吋骞舵э紝HD = C, NG = T, NI = A, NS = A,C, G, or T, NN = A or G, IG = T.杩欐牱鎴戜滑灏卞彲浠ラ娴嬪嚭涓浜沞ffector铔嬬櫧鎵璋冩帶鐨勯澏鍩哄洜锛屽苟涓旇繖椤瑰彂鐜板浠ュ悗鐮旂┒鍜岀敓鐗╂妧鏈殑寮鍙戝叿鏈夐噸瑕佷綔鐢紒
]]>The Rice Yellow Mottle Virus P1 protein exhibits dual functions to suppress and activate gene silencing.聽聽 [涓嬭浇]
棰樼洰涓湁Rice锛屼絾骞朵笉鍙槸鍏充簬姘寸ɑ鐨勩
siRNA鏄按绋绘姉鐥呮瘨鐨勪竴绉嶅唴鍦ㄦ満鍒讹紝鐩稿簲鐨勶紝鐥呮瘨涔熶細浜х敓鎶戝埗瀛愭姷鎶楄繖绉嶆矇榛橀斿緞銆傛按绋婚粍鏂戦┏鐥呮瘨(RYMV)涓殑P1铔嬬櫧锛岄氳繃鍑忓皯21nt鎴栬24nt siRNA鐨勭Н绱(姣斿鍦ㄦ按绋讳腑锛屼富瑕佸奖鍝岲CL4閫斿緞)锛屽鍩哄洜娌夐粯鏈夐樆鎶戞晥搴(涓嶅悓鏉ユ簮鐨凱1铔嬬櫧鏁堝簲鏈夎緝澶у樊鍒)銆備笉杩囧湪鐑熻崏涓殑寮傛簮琛ㄨ揪鍙戠幇锛岃铔嬬櫧鍙嶈屼績杩涙矇榛樹俊鍙风殑浼犲銆傛渶鍚庤В閲婂埌杩欒泲鐧借捣涓涓濆钩琛♀滅殑浣滅敤锛岃鐥呮瘨灏藉绻佹畺鐨勫悓鏃朵篃涓瀹氱▼搴︿笂淇濇姢瀹夸富銆
鍡紝閫犵墿涓荤湡绁炲鈥︹
In plant, RNA silencing is a host defense mechanism against viral infection in which double strand RNA is processed into 21-24nt short interfering RNA (siRNA). Silencing spreads from cell-to-cell and systemically through a sequence-specific signal to limit the propagation of the virus. To counteract this defense mechanism, viruses encode suppressors of silencing. The P1 protein encoded by the Rice yellow mottle virus (RYMV) displays suppression activity with variable efficiency according to the isolates they originated from. Here, we show that P1 proteins from two RYMV isolates displaying contrasting suppression strength reduced local silencing induced by single strand and double strand RNA in Nicotiana benthamiana leaves. This suppression was associated with a slight and a severe reduction in 21nt and 24nt siRNA accumulation, respectively. Unexpectedly, cell-to-cell movement and systemic propagation of silencing were enhanced in P1 expressing Nicotiana plants. When transgenically expressed in rice, P1 proteins induced specific deregulation of DCL4-dependent endogenous siRNA pathways whereas the other endogenous pathways were not affected. Since DCL4-dependent pathways play a key role in rice development, expression of P1 viral proteins was associated to the same severe developmental defects in spikelets as in dcl4 mutants. Overall, our results demonstrate that a single viral protein display multiple effects on both endogenous and exogenous silencing not only in a suppressive but also in an enhancive manner. This suggests that P1 proteins play a key role in maintaining a subtle equilibrium between defense and counter-defense mechanisms to insure an efficient virus multiplication and the preservation of host integrity.