PMID: 20085706 miRNA 鐢插熀鍖
Cell, Volume 140, Issue 1, 111-122, 8 January 2010
Transcriptional control of gene expression by microRNAs 
鎴戜滑鐭ラ亾miRNA鍜屽叾浠栧皬RNA姣斿siRNA涓鏍凤紝鏄湪杞綍鍚庤皟鎺у熀鍥犺〃杈撅紝浣嗘槸miRNA鍦ㄨ浆褰曟按骞充笂鏄惁璧峰埌璋冩帶浣滅敤涓嶆竻妤氾紝杩欑瘒鏂囩珷浣滆呭彂鐜帮紝鍦ㄥ皬绮掔钘撴暡闄pDCL1b锛圖ICER-LIKE1b锛夌殑绐佸彉浣撲腑锛宮iRNA鐨勭敓鐗╁悎鎴愪笉鍙楀奖鍝嶏紝浣嗘槸鍓垏闈跺熀鍥犵殑鍔熻兘涓уけ锛岀粨鏋滃鑷翠簡闈跺熀鍥犺浆褰曟湰鐨勪笅闄嶃傝繖绉嶇獊鍙樹綋鑱氶泦浜嗗ぇ閲忕殑miRNA:target-RNA澶嶅悎浣擄紝瀵艰嚧浜嗛澏鍩哄洜鐨勮秴鐢插熀鍖栵紝杩涜屼骇鐢熸姂鍒跺熀鍥犺〃杈剧殑鏁堟灉銆傝繖鏉￠氳矾涔熷彂鐢熷湪閲庣敓鍨嬪彈婵绱犲鐞嗘椂锛岃繖浜涚粨鏋滆〃鏄庯紝閫氳繃鐢插熀鍖栬捣濮嬫姂鍒跺熀鍥犺〃杈句緷璧栦簬miRNA 鍜屼粬鐨則arget RNA鐨勬瘮渚嬨傝繖涓彂鐜版剰涔夎繕鏄緢閲嶅ぇ鐨勶紝涔嬪墠璁や负鍦ㄦ鐗╁綋涓紝鐢插熀鍖栧彧鏄笌siRNA鏈夊叧銆
MicroRNAs (miRNAs) control gene expression in animals and plants. Like another class of small RNAs, siRNAs, they affect gene expression posttranscriptionally. While siRNAs in addition act in transcriptional gene silencing, a role of miRNAs in transcriptional regulation has been less clear. We show here that in moss Physcomitrella patens mutants without a DICER-LIKE1b gene, maturation of miRNAs is normal but cleavage of target RNAs is abolished and levels of these transcripts are drastically reduced. These mutants accumulate miRNA:target-RNA duplexes and show hypermethylation of the genes encoding target RNAs, leading to gene silencing. This pathway occurs also in the wild-type upon hormone treatment. We propose that initiation of epigenetic silencing by DNA methylation depends on the ratio of the miRNA and its target RNA.
杩涘睍鐪熸槸蹇晩锝