PMID: 19805056 small RNA 琛ㄨ揪璋冩帶 寮傛簮澶氬嶄綋
Proc Natl Acad Sci U S A. 2009 Oct 5.
缇庡浗澶氫釜澶у鍒嗗瓙鐢熺墿鐮旂┒涓績鍜屼腑绉戦櫌閬椾紶鎵鑱斿悎 鍙戝湪PNAS涓 锛屾枃涓彁鍒颁簡涓涓湁瓒ｇ殑鍚嶅瓧鈥済enetic buffer鈥濓紝鏆備笖缈昏瘧鎴愨滈仐浼犵紦鍐插墏鈥濆惂銆
鍦ㄧ浉杩戠殑鐗╃鍜屼腑闂存潅浜ょ涓爺绌秙mall RNA 琛ㄨ揪澶氭牱鎬э紝鐮旂┒瀵硅薄涓轰袱绉嶆嫙鍏拌姤妞嶇墿Arabidopsis thaliana 鍜 A. arenosa.锛屽畠浠殑寮傛簮鍥涘嶄綋Arabidopsis suecica,缁煎悎璇ュ洓鍊嶄綋鐨勬潅浜ゆ牚绯诲悗浠(F(1) and F(7))涓婏紝缁撴灉鍙戠幇閲嶅鍜岃浆搴у瓙鐩稿叧鐨剆iRNA鍦ˋrabidopsis thaliana 鍜 A. arenosa涓〃杈惧樊寮傚緢澶э紝杩欑宸窛鍙樺寲鐨剆iRNA缇ゅ湪F1涓篃寰堟槑鏄炬ュ墽鍙樺寲锛屼絾鍦ˋrabidopsis suecica鍜孎7涓繚鎸佺ǔ瀹氾紝miRNA 鍜 tasiRNA鐨勫簭鍒楀湪绉嶉棿淇濆畧浣嗘槸琛ㄨ揪妯″紡鍦ㄥ紓婧愬洓鍊嶄綋鍙婂叾浜叉湰涓彉鍖栧緢澶с傘傘傛讳箣锛岀粨璁篻enetic buffer 灏辨槸褰撴潅浜ょ鍐呭彂鐢焔enomic shock鏃讹紝涓轰簡鏌撹壊璐ㄥ拰鍩哄洜缁勭殑鍜岃皭绋冲畾锛岀浉鍏崇殑siRNA缇ゅ彂鎸ヤ簡浣滅敤锛岃岃〃杈惧樊寮傜殑miRNA鍒欏紩瀵肩潃gene鍦ㄨ〃杈句笂鐨勫彉鍖栧拰鐢熸椿鍔涚殑閫傚簲鎬с
PS:鎯冲埌浠婂勾浣滅墿鎵閲屼篃鍙戜簡绡囧叧浜庡皬楹﹀拰鏌愪竴杩戞簮鐗╃small RNA鍒嗗竷姣旇緝鐨勬枃绔犲彂鍦‵unct Integr Genomics IF 3.8锛岀湅鏍峰瓙鏂囩珷涓嶅厜IDEA鍑哄僵锛屾悶澶栦氦鍜屽鍚堜綔涔熸槸鐜嬮亾銆
Small RNAs, including microRNAs (miRNAs), small interfering RNAs (siRNAs), and trans-acting siRNAs (tasiRNAs), control gene expression and epigenetic regulation. Although the roles of miRNAs and siRNAs have been extensively studied, their expression diversity and evolution in closely related species and interspecific hybrids are poorly understood. Here, we show comprehensive analyses of miRNA expression and siRNA distributions in two closely related species Arabidopsis thaliana and Arabidopsis arenosa, a natural allotetraploid Arabidopsis suecica, and two resynthesized allotetraploid lines (F(1) and F(7)) derived from A. thaliana and A. arenosa. We found that repeat- and transposon-associated siRNAs were highly divergent between A. thaliana and A. arenosa. A. thaliana siRNA populations underwent rapid changes in F(1) but were stably maintained in F(7) and A. suecica. The correlation between siRNAs and nonadditive gene expression in allopolyploids is insignificant. In contrast, miRNA and tasiRNA sequences were conserved between species, but their expression patterns were highly variable between the allotetraploids and their progenitors. Many miRNAs tested were nonadditively expressed (deviating from the mid-parent value, MPV) in the allotetraploids and triggered unequal degradation of A. thaliana or A. arenosa targets. The data suggest that small RNAs produced during interspecific hybridization or polyploidization serve as a buffer against the genomic shock in interspecific hybrids and allopolyploids: Stable inheritance of repeat-associated siRNAs maintains chromatin and genome stability, whereas expression variation of miRNAs leads to changes in gene expression, growth vigor, and adaptation.
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