PMID: 20010688 nc-RNA FLC 鍐疯瀵煎熀鍥犳矇榛樻満鍒
Nature 462, 799 (2009)
Cold-induced silencing by long antisense transcripts of an Arabidopsis Polycomb target 
FLC鏄帶鍒跺紑鑺辩殑閲嶈鍩哄洜锛屾鐗╀綋鍐呭瓨鍦ㄥぇ閲忕殑non-coding RNA锛岃繖浜沶cRNA鐨勪綔鐢ㄥ苟涓嶆竻妤氾紝鍦ㄦ涔嬪墠宸茬粡鍙戠幇浜咶LC鐨刟ntisense杞綍鏈紝骞朵笖鐭ラ亾鍦ㄦ寔缁殑鍐峰鐞嗘椂锛屾鐗╃殑FLC鍩哄洜浼氳Polycomb 瀹舵棌铔嬬櫧 PHD鈥揚RC2 complex鎵鎶戝埗锛岃繖灏辨槸鏄ュ寲浣滅敤銆傚湪杩欑瘒鏂囩珷閲岋紝浣滆呭彂鐜板湪鎸佺画鍐峰鐞嗘儏鍐典笅锛孎LC鍩哄洜浼氫骇鐢熷ぇ閲忕殑antisense闀胯浆褰曟湰锛岃屾涔夎浆褰曟湰涓嬮檷锛屽苟涓旇繖涓庢槬鍖栦綔鐢ㄧ殑涓浜沵arker鍩哄洜鏃犲叧銆傜敤FLC鍩哄洜鐨刟ntisense杞綍鏈殑鍚姩瀛愬姞鍦ㄤ竴涓35S-GFP鐨勪笅娓革紝鍙戠幇鍦ㄥ喎澶勭悊涓婫FP涔熶細琚姂鍒躲備綔鑰呯殑缁撴灉璇存槑浜嗗湪鍐疯瀵间笅锛孎LC鍩哄洜浜х敓澶ч噺鐨勫弽涔夎浆褰曟湰锛岃繖涓杩囩▼鍦‵LC鐨勬矇榛樻棭鏈熻捣鍒伴噸瑕佷綔鐢紝杩涜屾嫑鍕烶olycomb鏈哄埗銆傚苟涓旇繖绉嶆満鍒朵篃璁告槸涓绉嶆櫘閬嶆満鍒讹紝鍦ㄧ壒娈婃椂鏈熸垨鏉′欢涓嬶紝閫氳繃鍙嶄箟鐨勮浆褰曟湰鏉ヨ皟鑺傛涔夎浆褰曟湰鐨勮〃杈俱
Transcription in eukaryotic genomes generates an extensive array of non-protein-coding RNA, the functional significance of which is mostly unknown1. We are investigating the link between non-coding RNA and chromatin regulation through analysis of FLC 鈥斺塧 regulator of flowering time in Arabidopsis and a target of several chromatin pathways. Here we use an unbiased strategy to characterize non-coding transcripts of FLC and show that sense/antisense transcript levels correlate in a range of mutants and treatments, but change independently in cold-treated plants. Prolonged cold epigenetically silences FLC in a Polycomb-mediated process called vernalization2. Our data indicate that upregulation of long non-coding antisense transcripts covering the entire FLC locus may be part of the cold-sensing mechanism. Induction of these antisense transcripts occurs earlier than, and is independent of, other vernalization markers3 and coincides with a reduction in sense transcription. We show that addition of the FLC antisense promoter sequences to a reporter gene is sufficient to confer cold-induced silencing of the reporter. Our data indicate that cold-induced FLC antisense transcripts have an early role in the epigenetic silencing of FLC, acting to silence FLC transcription transiently. Recruitment of the Polycomb machinery then confers the epigenetic memory. Antisense transcription events originating from 3鈥 ends of genes might be a general mechanism to regulate the corresponding sense transcription in a condition/stage-dependent manner.
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