Rice Papers

Genome Res. 2009 Aug

Clusters and superclusters of phased small RNAs in the developing inflorescence of rice 锛圥DF)

genome research 杩戝嚑骞村彂浜嗕笉灏戠瘒鍏充簬small RNA锛圡iRNA锛夌殑鏂囩珷锛屾柊鐨勫皬RNA锛屼互鍙婂湪鍩哄洜缁勫唴鐨勫垎甯� 瑙勫緥鎴栬�呭仛绉嶉棿绉嶅唴姣旇緝鐮旂┒锛岄兘鑴变笉寮�涓板瘜鐨勫熀鍥犵粍瀛︾煡璇嗭紝瀵规按绋诲皬RNA浠庡垵鏈熷埌瀵瑰悇涓粍缁囧悇涓椂鏈熷缓搴撳垎鏋愶紝杩欏凡缁忔槸绗琋绡囦簡锛岄鐩鐨勬槸鑺辨湡鐨勫皬RNA锛岋紙鍏跺疄鍟ラ兘鍋氫簡锛夛紝siRNA鏃╂湡涔熸湁浜哄仛杩囩皣鐘跺垎甯冪殑鐮旂┒锛屼絾鏄偅鏃跺�欏鏁翠釜small RNA鐨勪簡瑙ｈ繕娌￠偅涔堜赴瀵屽锛岀幇鍦ㄥ洖杩囧ご鏉ュ啀鍒嗘瀽涓�鐣紝閰嶄笂婕備寒鐨勫浘鐗囷紝鍙堟槸涓�绡囧ソ鏂囥��

涓轰簡鐮旂┒姘寸ɑ鍙戣偛杩囩▼涓璼mall RNA鐨勮皟鑺備綔鐢紝瀵规垚鐔熷彾鐗囷紝鏍癸紝鑼庡拰鑺眘mall RNA鍋�454娴嬪簭鍒嗘瀽锛堟湁閽辨湁鎶�鏈晩锛侊級锛宑luster澶氱敱涓ょsmall RNA缁勬垚24-nt RNA鍜�21锛峮tRNA锛屽畠浠殑鍒嗗竷鍜岃〃杈炬ā寮忛兘涓嶄竴鏍枫��24锛峮tRNA瀵岄泦鍦ㄥ熀鍥犵粍鐨勯噸澶嶅尯鍩熷苟涓斿湪鍥涚缁勭粐閮借〃杈句絾鏄尯鍒笉澶э紝鏂囩珷缁欑殑缁撹鏄�24锛峮t RNA瀵归噸澶嶅尯鍏冧欢鐨勬矇榛樿捣鐫�housekeeping 聽鐪嬪鍔熻兘锛岃��21nt RNA缁勬垚铏藉皯锛屼笖鍦ㄥ熀鍥犵粍鍒嗗竷涓婇潪闅忔満锛� 鍗磋兘琚垎鎴�31 superclusters,锛坢iRNA鏋滅劧灏忛褰撳锛夛紝杩欑被閲嶅鍜宻mall RNA鐨勫舰鎴愮浉鍏筹紝骞朵笖浼樺厛鍦ㄨ姳鏈熻〃杈撅紝鍏朵腑杩樺寘鎷竴閮ㄥ垎閫�鍖栫殑22nt RNA锛岀爺绌惰繕鍙戠幇涓�绫绘柊鍨媘iRNA鍙兘浠嬪cleavage of the cluster motif銆傘�傘��

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